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. 2021 Sep 8;8:725072. doi: 10.3389/fmed.2021.725072

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Copyright © 2021 De Meyer, Fijalkowski, Jonckheere, De Sutter, Eyckerman and Van Damme.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of Gag-SspH2 virus-like particles and interaction with profilin. Virotrap control Gag-eDHFR and S. Typhimurium effector Gag-SspH2 fusion was co-expressed with FLAG-tagged vesicular stomatitis virus glycoprotein (VSV-G) in HEK293T cells. (A) For both setups, virus-like particles (VLPs) were captured from the cell medium using anti-FLAG immunoprecipitation. VLP lysates loaded on gel represent a 20-fold equivalent of the producer cell lysate loaded. (B) Reciprocal co-immunoprecipitation was done with producer cell lysates of cells expressing Gag-eDHFR and Gag-SspH2 using anti-Gag and anti-PFN1 antibodies. The asterisk indicates a non-specific/degradation signal.