Table 1.
Overview of literature confirmed SspH2 interactors.
| Host protein | Model system | Info | Tag orientation | Method | Publication |
|---|---|---|---|---|---|
| SUGT1 | HEK293T cells | Overexpression bait | N-terminal (HA) | SILAC/AP-MS | (14) |
| Overexpression bait | N-terminal (HA) | co-IP | |||
| HeLa cells | Infection context | N-terminal (HA) | SILAC/AP-MS | (15) | |
| NOD1 | HeLa cells | Overexpression bait and prey | bait N-terminal (HA), prey C-terminal (FLAG) | co-IP | (9) |
| PFN1 | Yeast | Ectopical expression bait and prey | bait N-terminal (Gal4BD), prey N-terminal (Gal4AD) | Y2H | (16) |
| HEK293T cells | Overexpression bait | N-terminal (GST) | co-IP | ||
| HeLa cells | Infection context | N-terminal (HA) | SILAC/AP-MS | (15) | |
| HEK293T cells | Overexpression bait | N-terminal (Gag) | Virotrap | This study | |
| PFN2 | HeLa cells | Infection context | N-terminal (HA) | SILAC/AP-MS | (15) |
| HEK293T cells | Overexpression bait | N-terminal (Gag) | Virotrap | This study | |
| FLNA | Yeast | Ectopical expression bait and prey | bait N-terminal (Gal4BD), prey N-terminal (Gal4AD) | Y2H | (16) |
| HeLa cells | Infection context | N-terminal (HA) | SILAC/AP-MS | (15) |
HEK293T, human embryonic kidney 293T; HA, Hemagglutinin; SILAC, stable isotope labeling with amino acids in cell culture; AP-MS, affinity purification mass spectrometry; co-IP, co-immunoprecipitation; Y2H, yeast-two-hybrid.