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. 2021 Sep 8;9:659742. doi: 10.3389/fbioe.2021.659742

FIGURE 2.

FIGURE 2

Effect of PRP on cell osteogenic differentiation of hDPCs was assessed by ALP, alizarin red staining, and real-time quantitative PCR. (A) Cells cultured in the presence of different concentrations of PRP were fixed and stained for ALP (scale bar, 50 μm). (B) Cells were fixed and stained with alizarin red (scale bar, 50 μm). (C) Real-time quantitative-PCR analysis of ALP, COL 1, OCN, DMP-1, DSPP mRNA expression in different groups. Data in (C) are representative of five independent experiments. Data are presented as mean ± standard deviation. Significantly different groups, *p < 0.05, **p < 0.001.