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. 2021 Sep 8;9:700862. doi: 10.3389/fbioe.2021.700862

FIGURE 4.

FIGURE 4

Expanded CD133+ cell cycle and proliferation after the culture on the PLA scaffolds. (A) Percentage of expanded CD133+ cells in each of the phases of the cell cycle after 48 h of culture on the scaffolds in comparison to the culture on the PLA disc and the control (Control 2D n = 6; PLA disc n = 3; S25 n = 8; S40 n = 8). Analysis by one-way ANOVA: a p ≤ 0.05 in comparison to the control 2D; c p ≤ 0.001 in comparison to the control 2D; d p ≤ 0.05 in comparison to the PLA disc; e p ≤ 0.01 in comparison to the PLA disc (B) Cell proliferation analysis by EdU incorporation after 48 h of culture on the 3D scaffold in comparison to the control 2D (Control 2D n = 2; 3D n = 3) (C) Percentage of cells in each of the phases of the cells cycle after 48 h and 7 days of culture on the S25 (S25 7 days n = 5) (D) and on the S40 (S40 7 days n = 4) (E) Comparison of the percentage of cells in each of the phases of the cell cycle after 7 days of culture on the S25 and S40. For the 3D scaffolds n represents the number of scaffolds that were analyzed. Graphs are represented by the mean ± SD. Student’s unpaired t-test analysis: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.