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. 2021 Sep 8;9:714996. doi: 10.3389/fcell.2021.714996

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Copyright © 2021 Liu, Liu, Wang, Wan, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of Keap1 in Dex-treated CCI-induced rats intrathecally injected with lentiviral-NC or lentiviral-Keap1 for 1 week. Rats were then subjected to Dex treatment and CCI surgery. (A,B) mRNA expression levels of Keap1 and Nrf2 was measured using qPCR. (C) mRNA expression level of HO-1 in the spinal cord was detected using qPCR. (D) Isolated microglia were transfected with ARE luciferase vectors, and luciferase activity was determined in the cells at 36 h after transfection. (E) Protein expression levels of Nrf2, Keap1, and HO-1 were measured using WB. (F) Immunohistochemical staining of Keap1 and Nrf2 expression in spinal cord tissues. Scale bar, 50 μm. *P < 0.05, **P < 0.01 vs. CCI group; ^#P < 0.05, ^##P < 0.01 vs. CCI+Dex group.