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. 2021 Sep 8;9:714996. doi: 10.3389/fcell.2021.714996

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Copyright © 2021 Liu, Liu, Wang, Wan, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Keap1 knockdown in CCI-induced rats intrathecally injected with lentiviral-shNC or lentiviral-shKeap1 for 1 week. Rats were then subjected to CCI surgery. (A,B) Expression levels of Keap1 and Nrf2 in the spinal cord were measured using qPCR. (C) mRNA expression level of HO-1 in the spinal cord was assayed using qPCR. (D) Isolated microglia were transfected with ARE luciferase vector, and luciferase activity was measured at 36 h after transfection. (E) Protein expression levels of Keap1, Nrf2, and HO-1 were determined using western blotting. (F) Immunohistochemical staining for Keap1 and Nrf2 expression. Scale bar, 50 μm. **P < 0.01 vs. Sham group; ^##P < 0.01 vs. CCI+shNC group.