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. 2021 Sep 8;9:714996. doi: 10.3389/fcell.2021.714996

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Copyright © 2021 Liu, Liu, Wang, Wan, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Keap1 silencing on CCI-repressed antioxidant capacity in spinal cord tissues of rats. Rats were intrathecally injected with lentiviral-shNC or lentiviral-shKeap1 for 1 week and then subjected to CCI surgery. (A) Levels of antioxidant genes in the spinal cord were determined via quantitative polymerase chain reaction. (B) Analysis of glutathione levels. (C) A superoxide dismutase assay kit was used to examine SOD activity. (D) Determination of catalase activity. *P < 0.05, **P < 0.01 vs. Sham group; ^#P < 0.05, ^##P < 0.01 vs. CCI+shNC group.