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. 2021 Sep 8;12:739849. doi: 10.3389/fgene.2021.739849

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Copyright © 2021 Yang, Xu, Lin, Chen, Lin, Song, Bai, You and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression levels of differentially expressed miRNAs in midguts of the resistant (Cry1S1000) and susceptible (G88) strains by RT-qPCR (A), and up-/down-regulated expression of the miRNAs based on Illumina sequencing (B). The expression levels of miRNAs were normalized by U6. Statistical significance was analyzed using one-way ANOVA. The asterisks represent significance, where two asterisks indicate p < 0.01, and one asterisk indicates p < 0.05. (B) The orange column above the X-axis shows up-regulated miRNAs, while the purple column below the X-axis shows down-regulated miRNAs based on Illumina sequencing.