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. 2021 Sep 8;8:708008. doi: 10.3389/fvets.2021.708008

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Copyright © 2021 Yin, Bai, Wu, Li, Shao, Sun and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE analysis of the fusion protein. (A) Protein expression results. M, marker; lane 1, whole bacteria after ultrasound; lane 2, supernatant after ultrasound; lane 3, precipitation after ultrasound. (B) SDS-PAGE after protein purification. M, marker; lane 1, the original protein before purification; lane 2, flow-through solution; lane 3, 15 mm of imidazole elution fraction; lane 4, 60 mm of imidazole elution fraction; lane 5, 300 mm of imidazole elution fraction.