FIGURE 4.

Neutrophils from healthy subjects pretreated with a vehicle (A,C,E,G) or RNO (100 nMoles/L) (B,D,F,H) were exposed to endotoxin and allowed to adhere on fibrinogen-coated surfaces for 18 h in the absence (C,D) or in the presence (G,H) of CFTRinh-172 (10 µMoles/L). Unstimulated neutrophils were incubated in parallel in the absence (A,B) or in the presence (E,F) of CFTRinh-172 (10 µMoles/L). At the end of the incubation, samples were stained for intracellular myeloperoxidase and analyzed by flow cytometry. Intact neutrophils were identified on the basis of typical SSC and FSC and analyzed for myeloperoxidase content. Results are from one representative experiment MV (neutrophil microvesicles).