FIGURE 5.

Neutrophils isolated from healthy subjects (n = 16) (A,C) or from volunteers with CF (n = 7) (B,D) were treated with RNO (100 nMoles/L) (shaded bars) or a vehicle (white bars) and allowed to adhere on fibrinogen-coated surfaces in the presence or absence of CFTRinh-172 (10 μMoles/L), for 18 h with or without endotoxin. At the end of the incubation, the percentage of intact cells (A,B) and annexin V binding related to intact cells (C,D) was evaluated by flow cytometry (Figure 4). Intact neutrophils were identified by typical SSC and FSC. *p < 0.05 (Student’s t test) vs vehicle-treated samples.