FIGURE 6.
C57BL/6 male mice (8–10 weeks of age) were infected i.t. with 1 × 106 CFUs of MDR-RP73 embedded in agar beads and per aerosol with roflumilast (5 mg/kg) or placebo (4,4% DMSO in saline) once a day starting from 4 h after infection. Animals were killed after 28 h or 5 days of infection, and the BALF was collected. Total cells (A), neutrophils (B), and macrophages (C) were counted in the BALF. Panels show box plots of cell numbers at the time of killing(28 h and 5 days after infection) of vehicle- (n = 9 per group) or roflumilast-treated mice (n = 9 killed at 28 h and n = 8 killed at 5 days after infection). The horizontal lines mark the median of values, the edges of each box mark the 25th and 75th percentiles, and the vertical lines indicate the highest and lowest values, respectively, which are not outliers (values greater than 1.5 times the length of the box were considered outliers and excluded from the analysis). *p < 0.05 (ANOVA, Dunnett’s test) vs vehicle-treated mice.