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. 2021 Aug 21;62:100109. doi: 10.1016/j.jlr.2021.100109

Fig. 4.

Fig. 4

12-HETE-esterified in PLs of HT29 cells pre-exposed to platelets and in platelets. A: In the cell pellet of HT29 cells pre-exposed to platelets (HT29 from HT + PLT) and of platelets cultured alone (PLT), the levels of PE18:0a_12-HETE, PE18:0p_12-HETE, PE16:0p_12-HETE, PE18:1p_12-HETE, PC16:0a_12-HETE, and PC18:0a_12-HETE were assessed by LC-MS/MS; data are reported as the total amount (ng), all data are shown as scatter dot plots with mean + SEM, n = 6, 7; ∗∗P < 0.01 versus the same PL detected in HT29 cells; §P < 0.01 versus PE18:0p_12-HETE and PE16:0p_12-HETE of HT29 cells (using one-way ANOVA and Tukey's multiple comparisons test). B: The percentage of 12-HETE esterification to total 12-HETE (i.e., free and esterified forms) was calculated in HT29 cells pre-exposed to platelets (HT) and in platelets alone (PLT); all data are shown as scatter dot plots with mean + SEM, n = 6, 7; ∗∗P < 0.01 versus PLT (unpaired t test, two-tailed).