Fig. 1.

Inhibition of endothelial lipase by ANGPTL3. A: Phospholipase activity of EL after incubation with increasing concentrations of ANGPTL3 for 30 min at 37°C. Activity was normalized to control-treated EL. Points represent mean (±SD) of three independent experiments; each performed with biological duplicates. B and C: Phospholipase activity over time of EL incubated with or without 2 μg/ml ANGPTL3. Activity was normalized either to the activity of EL without ANGPTL3 (control treated) at time point 0 (B) or to the respective control-treated EL for each time point (C). Points represent mean (±SD) of three independent experiments; each performed with biological duplicates. ANGPTL3, angiopoietin-like 3; EL, endothelial lipase.