FIG. 4.
(Top panel) IL-3 induction of mcl-1 expression is attenuated by PI3-K inhibitors. Ba/F3 cells deprived of cytokine were pretreated with various inhibitors as indicated for 30 min prior to stimulation with IL-3 for 1 h. After stimulation, total RNA was prepared from these cells and analyzed by Northern blotting by using a 32P-labeled probe specific for detection of the murine mcl-1 mRNA. (Bottom panel) The same RNA samples stained with ethidium bromide before probing.