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. 1999 Sep;19(9):6195–6206. doi: 10.1128/mcb.19.9.6195

FIG. 4.

FIG. 4

(Top panel) IL-3 induction of mcl-1 expression is attenuated by PI3-K inhibitors. Ba/F3 cells deprived of cytokine were pretreated with various inhibitors as indicated for 30 min prior to stimulation with IL-3 for 1 h. After stimulation, total RNA was prepared from these cells and analyzed by Northern blotting by using a 32P-labeled probe specific for detection of the murine mcl-1 mRNA. (Bottom panel) The same RNA samples stained with ethidium bromide before probing.