REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Mammary gland carcinoma tissue	Mouse models from Australian BioResources (MossVale, NSW, Australia)	N/A
Antibodies
PE/Cyanine7 anti-mouse CD45 (Dilution 1:400)	BioLegend	Cat#103114; RRID: AB_312979
Chemicals, peptides, and recombinant proteins
Collagenase	Sigma Aldrich	Cat#C9891; CAS: 9001-12-1
Hyaluronidase	Sigma Aldrich	Cat#H3506; CAS: 37326-33-3
Ammonium chloride	Sigma Aldrich	Cat#A9434; CAS: 12125-02-9
DNase I	Roche	Cat#10104159001
2.5% Trypsin	Gibco	Cat#15090-046
Dispase II (neutral protease, grade II)	Roche	Cat#04942078001
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA)	Sigma	Cat# E3889; CAS: 67-42-5
Polyvinyl alcohol	Sigma	Cat# P8136; CAS: 9002-89-5
DMEM High Glucose	Gibco	Cat# 11995-073
MEM, Hanks' Balanced Salts	Gibco	Cat# 12571063
Dulbecco's phosphate-buffered saline (DPBS)	Gibco	Cat# 14190-250
Fetal Bovine Serum (FBS)	GE Healthcare	Cat# SH30406.02
4',6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI)	Invitrogen	Cat# D1306; RRID: AB_2629482
Trypan Blue Solution, 0.4%	ThermoFisher Scientific	Cat# 15250061
Critical commercial assays
Dead Cell Removal Kit	Miltenyi Biotec	Cat#130-090-101
Experimental models: Cell lines
4T1.2	Gifted from Prof. Robin Anderson (Olivia Newton-John Cancer Research Institute)	N/A
67NR	Gifted from Prof. Robin Anderson (Olivia Newton-John Cancer Research Institute)	N/A
Experimental models: organisms/strains
Female BALB/c mice intraductally injected at 8–10 weeks old with the 4T1.2 or 67NR cell lines	Inbred strain from Australian BioResources (origin The Jackson Laboratory)	Cat# JAX:000651; RRID:IMSR_JAX:000651
Mammary tumor virus-Polyoma Middle T (MMTV-PyMT) model of luminal breast cancer (females aged between 12–14 weeks old)	Inbred strain from Australian BioResources (origin The Jackson Laboratory)	Cat# JAX:002374; RRID:IMSR_JAX:002374
Software and algorithms
FlowJo v10.6.2	Becton Dickinson	https://flowjo.com
Other
BD FACSAria™ III sorter	BD Biosciences	N/A
McIlwain Tissue chopper	ProSciTech	Cat# U0800
Incu-Shaker™ 10 L Shaking Incubator	Benchmark Scientific	Part#: 6.284 19
Hemocytometer	Sigma-Aldrich	Cat# Z359629