Functional characterization of wild-type and mutant heteromeric GluK2 KARs co-assembled with either GluK5 or Neto2
(A) Representative whole-cell currents evoked by a 1 s application of glutamate (10 mM, gray bar) from heteromeric GluK5-containing receptors assembled with the indicated GluK2 subunits expressed in HEK293-T/17 cells.
(B–D) Quantitation of the peak amplitude, desensitization, and percent desensitization for GluK2/GluK5 receptors.
(E) Representative whole-cell currents evoked by a 1 s application of glutamate (10 mM, gray bar) from wild-type and mutant GluK2/Neto2 receptors expressed in HEK293-T/17 cells.
(F–H) Quantitation of the peak amplitude, desensitization, and percent desensitization for GluK2/Neto2 receptors. n = 7 [GluK2/Neto2], 8 [GluK2(T660K)/Neto2], 5 [GluK2(T660R)/Neto2], and 11 [GluK2(I668T)/Neto2]. KAR, kainate receptor; Neto2, Neuropilin- and tolloid-like protein 2; WT, wild-type; wtd, weighted. Statistical significance is denoted as ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Error bars represent SEM.