Figure 3.

dTnpo is highly expressed in neurons, including mushroom body neurons

The dTnpo CRIMIC (T2A-GAL4) allele was used to drive expression of UAS-fluorescent reporter transgenes.

(A–L) UAS-mCherry.NLS (nuclear mCherry) was expressed and tissue were dissected from L3 larvae (CNS, includes central brain and VNC) or adults (brain). Shown is half of the adult brain. Tissue were counterstained with markers for neurons (Elav) or glia (Repo). Z stacked images showing dTnpo expression pattern compared to neurons (A–F) or glia (G–L). Dashed squares indicate regions used in (A′)–(L′).

(A′–L′) Single slice images were used to better visualize cellular co-localization of mCherry.NLS signal with neurons or glia. White arrows highlight co-localized nuclei with most neurons and some glia.

(M–R) dTnpo CRIMIC driven expression of UAS-mCD8::RFP (membrane-bound RFP) and FasII counter-staining confirmed overlap of dTnpo expression and mushroom body (MB) neurons in both larval and adult brains.

(S and T) Schematics of the larval CNS (S) and adult brain (T) highlighting MB neurons (blue), the ventral nerve cord (VNC), the central brain, and optic lobes (OL). The adult OL includes the medulla and lamina. The adult brain also includes the subesophageal ganglion (not shown in the schematic).