Figure 5.

Upregulation of dTnpo disrupts morphology of eyes and wings

(A) Ubiquitous expression of UAS::dTnpo^GS11030 using da-GAL4 in flies causes a 25-fold increase in dTnpo mRNA levels by qPCR. L3 larvae were analyzed at 22°C. Unpaired t test, ^∗∗∗p = 0.0003. Each dot represents the mean from replicate wells per sample. The mean from 4 individual samples is shown. Error bars denote SD.

(B) da-GAL4>UAS::dTnpo^GS11030 animals do not survive beyond pupariation at 25°C.

(C and D) Upregulation of dTnpo during eye development, using either ey-GAL4 (early development) or GMR-GAL4 (late development) driven expression of UAS::dTnpo^GS11030, causes small eyes and rough eye phenotypes. Scale bar = 100 μm.

(E) nub-GAL4 driven expression of UAS::dTnpo^GS11030 causes notch and blister phenotypes (arrows) in the fly wing. Scale bar = 200 μm. “Control (Ctrl)” is UAS-empty.