Figure 5.

Hierarchical clustering of activity and abundance scores and cluster accessibility

(A–D) In (A), dendrogram and heatmaps of CYP2C9 activity and abundance score clustered by position. Heatmaps colored as in Figure 4. Only positions that had at least 26 total substitutions were included in this analysis. Colored boxes on the left indicate the six major clusters and correspond to the colors shown in (B), (C), and (D). In (B) and (C), the positions that correspond to each of the six clusters are shown as spheres in the corresponding color on the CYP2C9 crystal structure (PDB: 1R9O). Alternate viewpoint is shown in (C). In (D), relative solvent accessibility of each cluster is shown as a boxplot. Bold black line shows median, box shows 25th and 75th percentile, vertical line shows 1.5 interquartile range above and below percentiles, and outliers are shown as black points.