Figure 2.

(Top) Z-projections of Atto488-LacY GUVs showing protein increase. (Bottom) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ^∗Quantification by the biochemical Markwell-Lowry assay in LUVs. ^∗∗Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.