Table 2.
Fluorescence lifetimes (in nanoseconds) of C15X-L-PA in DPPC respective amplitudes (in parentheses) from 10 to 60°C and back
| C151-L-PA |
C152-L-PA |
|||
|---|---|---|---|---|
| temp. (°C) | Buffer τ1 | Buffer τ1 (A1) | Polar τ2 (A2) | Nonpolar τ3 (A3) |
| 10 | 4.90 | 0.55 (0.54) | 1.44 (0.20) | 4.21 (0.26) |
| 20 | 4.87 | 0.43 (0.51) | 1.45 (0.30) | 4.10 (0.19) |
| 30 | 4.81 | 0.35 (0.50) | 1.41 (0.37) | 4.10 (0.13) |
| 36 | 4.85 | 0.32 (0.42) | 1.35 (0.48) | 4.34 (0.10) |
| 42 | 4.97 | 0.29 (0.29) | 1.28 (0.64) | 4.56 (0.07) |
| 50 | 5.02 | 0.18 (0.17) | 0.98 (0.78) | 5.10 (0.05) |
| 60 | 4.90 | 0.17 (0.21) | 0.77 (0.74) | 5.02 (0.05) |
| 50 | 5.04 | 0.17 (0.17) | 0.97 (0.78) | 5.01 (0.05) |
| 42 | 5.07 | 0.26 (0.24) | 1.25 (0.69) | 4.40 (0.07) |
| 36 | 4.89 | 0.31 (0.40) | 1.32 (0.50) | 4.25 (0.10) |
| 30 | 4.87 | 0.34 (0.44) | 1.41 (0.44) | 4.14 (0.12) |
| 20 | 4.85 | 0.41 (0.47) | 1.53 (0.35) | 4.16 (0.18) |
| 10 | 4.90 | 0.53 (0.51) | 1.45 (0.23) | 4.27 (0.26) |
Amplitudes have been corrected for their respective radiative rates. Lifetime uncertainties are ±0.2 ns; amplitude uncertainties are ±0.04.