Skip to main content
. 2021 Jul 24;120(17):3676–3687. doi: 10.1016/j.bpj.2021.07.021

Table 4.

Fluorescence lifetimes (in nanoseconds) of C15X-NAT in DPPC respective amplitudes (in parentheses) from 10 to 60°C and back down to 10°C


C151-NAT
C152-NAT
temp. (°C) Buffer τ1 (A1) Complex τ2 (A2) Buffer τ1 (A1) Polar τ2 (A2) Nonpolar τ3 (A3)
10 4.12 (0.89) 0.63 (0.11) 0.52 (0.63) 1.63 (0.22) 4.16 (0.15)
20 3.98 (0.90) 0.66 (0.10) 0.43 (0.60) 1.42 (0.28) 3.90 (0.12)
30 3.87 (0.89) 0.72 (0.11) 0.39 (0.57) 1.51 (0.35) 3.69 (0.08)
36 3.84 (0.88) 0.77 (0.12) 0.36 (0.53) 1.42 (0.40) 3.62 (0.07)
42 4.06 (0.85) 0.70 (0.15) 0.34 (0.22) 1.27 (0.74) 3.72 (0.04)
50 3.90 (0.86) 0.78 (0.14) 0.23 (0.21) 0.98 (0.75) 3.86 (0.04)
60 3.66 (0.87) 0.73 (0.13) 0.21 (0.23) 0.76 (0.73) 3.48 (0.04)
50 3.86 (0.86) 0.70 (0.14) 0.23 (0.21) 0.97 (0.75) 3.58 (0.04)
42 4.07 (0.85) 0.75 (0.15) 0.29 (0.21) 1.22 (0.75) 3.75 (0.05)
36 3.82 (0.88) 0.76 (0.12) 0.34 (0.52) 1.38 (0.41) 3.67 (0.08)
30 3.88 (0.89) 0.77 (0.11) 0.38 (0.54) 1.47 (0.37) 3.75 (0.09)
20 4.02 (0.88) 0.72 (0.12) 0.41 (0.55) 1.45 (0.32) 3.88 (0.13)
10 4.14 (0.88) 0.65 (0.12) 0.45 (0.52) 1.41 (0.31) 4.14 (0.17)

Amplitudes have been corrected for their respective radiative rates. Lifetime uncertainties are ±0.2 ns; amplitude uncertainties are ±0.04.