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. 2021 Sep 14;24(5):797. doi: 10.3892/mmr.2021.12437

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — HOXC10 is upregulated in colorectal cancer cells, and binds to the MTFR2 promoter to activate MTFR2 expression. (A) mRNA and (B) protein expression levels of HOXC10 in HCT116 and HIEC-6 cell lines were analyzed by RT-qPCR and western blotting, respectively. ***P<0.001 vs. HIEC-6. (C) Binding sites between HOXC10 and MTFR2 were determined using the Japan Automotive Software Platform and Architecture program. (D) mRNA and (E) protein expression levels of HOXC10 in HCT116 cells transfected with Oe-HOXC10 were analyzed by RT-qPCR and western blotting, respectively. (F) mRNA and (G) protein expression of MTFR2 in HCT116 cells transfected with Oe-HOXC10 were analyzed by RT-qPCR and western blotting, respectively. *P<0.05 and ***P<0.001 vs. control group; ^#P<0.05 and ^###P<0.001 vs. Oe-NC group. (H) Relative luciferase activity was determined using a dual-luciferase reporter assay in HCT116 cells co-transfected with Oe-HOXC10 or Oe-NC and MTFR2-WT or MTFR2-MUT. ***P<0.001 vs. Oe-NC group. (I) Chromatin immunoprecipitation confirmed the interaction between HOXC10 and MTFR2. ***P<0.001 vs. IgG group. MTFR2, mitochondrial fission regulator 2; RT-qPCR, reverse transcription-quantitative PCR; Oe, overexpression; NC, negative control; WT, wild-type; MUT, mutant.