. Author manuscript; available in PMC: 2022 Sep 1.

Published in final edited form as: Radiat Res. 2021 Sep 1;196(3):235–249. doi: 10.1667/RADE-20-00286.1

TABLE 3.

Individual Senescent Cell Containing Colonies (Scored on Day 7 and Day 14 CFU-GEMM) from Freshly Explanted Marrow at 60 Days or One Year after 8 Gy TBI from p16^+/LUC compared to C57BL/6 mice

		Time after 8 Gy TBI
		Control	60 days	1 year
Day 7 CFU-GEMM
p16^+LUC	LUC⁺ colonies	2.3 ± 1.5	3.0 ± 1.1	2.3 ± 0.9
	No. of colonies	34.0 ± 5.0	37.0 ± 0.6	45.7 ± 3.5
C57BL/6	LUC⁺ colonies	0	0	0
	No. of colonies	60.7 ± 3.7	64.0 ± 1.5	33.7 ± 0.9
Day 14 CFU-GEMM
p16^+LUC	LUC⁺ colonies	2.7 ± 0.7	2.3 ± 1.5	2.7 ± 1.2
	No. of colonies	54.0 ± 4.0	34.0 ± 5.0 (P = 0.0363)	39.3 ± 2.6 (P = 0.0322)
C57BL/6	LUC⁺ colonies	0	0	0
	No. of colonies	46.7 ± 1.4	62.7 ± 2.3 (P = 0.043)	38.3 ± 2.2 (P = 0.0437)

Notes. Significance (P ≤ 0.05) compared to control is indicated in bold face. Cells were plated in CFU-GEMM colony assay as described in the Materials and Methods with four culture plates per data point. (1 × 10⁴ nucleated cell, plates per plate) (n = 4 plate per point). Results are presented as number of colonies ≥50 cells in 10⁴ cell plates. Number of senescent single cells were similar for marrow harvested at 60 days compared to one year after TBI.