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. 2021 Sep 8;22(5):1278. doi: 10.3892/etm.2021.10713

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-122-5p mimic further promotes LPS-treated acute lung injury cell injury. (A) RT-qPCR analysis of miR-122-5p expression in A549 cells transfected with mimic control or miR-122-5p mimic for 24 h. (B) RT-qPCR analysis of miR-122-5p expression in A549 cells in the different groups. (C) MTT assay of cell viability in the different groups. (D) LDH release analysis in the different groups of cells. (E) Flow cytometry analysis of apoptosis and (F) the apoptotic ratio. (G) Western blotting of cleaved-caspase 3 protein expression and the (H) cleaved-caspase 3/caspase 3 ratio. ^**P<0.01 vs. mimic control; ^##P<0.01 vs. control; ^&&P<0.01 vs. LPS + mimic control. miR, microRNA; LPS, lipopolysaccharide; RT-qPCR, reverse transcription-quantitative PCR; LDH, lactate dehydrogenase.