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. 2021 Sep 8;22(5):1278. doi: 10.3892/etm.2021.10713

Figure 5.

Figure 5

Transfection efficiency of miR-122-5p inhibitor and IL1RN-siRNA in A549 cells. (A) RT-qPCR analysis of miR-122-5p expression following A549 cell transfection with the inhibitor control or miR-122-5p inhibitor. (B) RT-qPCR analysis of IL1RN expression of A549 cells following transfection with control-siRNA or IL1RN-siRNA. (C) Western blotting and (D) RT-qPCR analysis of IL1RN expression in A549 cells following transfection with miR-122-5p inhibitor + control-siRNA or miR-122-5p inhibitor + IL1RN-siRNA. **P<0.01 vs. inhibitor control; ##P<0.01 vs. control-siRNA; &&P<0.01 vs. miR-122-5p inhibitor + control-siRNA. miR, microRNA; siRNA or si, small interfering RNA; IL1RN, IL-1 receptor antagonist; RT-qPCR, reverse transcription-quantitative PCR.