FIG. 6.

Complementation of hTR(58-451) with template-containing hTR truncations to assemble active telomerase. Assembly reactions for lanes 1 to 9 contained 10 ng of hTR(58-451) and either no additional RNA (lane 1) or a template-containing RNA present as the molar equivalent to 1 μg of full-length hTR, as indicated (lanes 2 to 9). Reactions for lanes 10 to 12 contain increasing quantities of hTR-wt(1-451): 10 ng (lane 10), 30 ng (lane 11) and 100 ng (lane 12). To compensate for differences in RNA quantities, 3.3 μg of yeast tRNA was included in each sample. A fraction (1/20) of the assembly reaction product was examined by the TRAP assay. Relative quantitation is shown for this representative TRAP assay and reflects the ratio between the abundance of extended products versus the 36-bp internal standard.