TABLE 1.
Primers for amplifying hTR sequences
| Primer | Description | Sequencea |
|---|---|---|
| T7HTR+1 | T7 promoter plus +1 to +20 | cgtaatacgactcactataGGGTTGCGGAGGGTGGGCCT |
| T7HTR+33 | T7 promoter plus +33 to +55 | cgtaatacgactcactatagGGCCATTTTTTGTCTAACCCTAA |
| T7HTR+44 | T7 promoter plus +44 to +63 | cgtaatacgactcactataggGTCTAACCCTAACTGAGAAG |
| T7HTR+58 | T7 promoter plus +58 to +74 | cgtaatacgactcactataggGAGAAGGGCGTAGGCGC |
| T7HTR+164 | T7 promoter plus +164 to +185 | cgtaatacgactcactatagggAGCAAACAAAAAATGTCAGCTG |
| T7HTR+206 | T7 promoter plus +206 to +221 | cgtaatacgactcactataGGGGACCTGCGGCGGG |
| T7HTR+250 | T7 promoter plus +250 to +265 | cgtaatacgactcactatagGGAGGCCGCGGTCGGC |
| T7HTRmt+33 | T7 promoter plus +33 to +64 | cgtaatacgactcactatagGGCCATTTTTTGTCaAACCCaAACTGAGAAGG |
| (TTTGGG template mutant) | ||
| HTR+451 | Complement of +432 to +451 | GCATGTGTGAGCCGAGTCCT |
| HTR+354 | Complement of +337 to +354 | CCTGAACCTCGCCCTCGC |
| HTR+325 | Complement of +306 to +325 | CCGCGGCTGACAGAGCCCAA |
| HTR+300 | Complement of +281 to +300 | CGCGGTGGCAGTGGGTGCCT |
| HTR+280 | Complement of +261 to +280 | CCGGAGAAGCCCCGGGCCGA |
| HTR+205 | Complement of +189 to +205 | GGGAGGGGCGAACGGGC |
| HTR+163 | Complement of +143 to +163 | CTAGAATGAACGGTGGAAGGC |
| HTR+147 | Complement of +130 to +147 | AAGGCGGCAGGCCGAGGC |
| HTR+104 | Complement of +87 to +104 | GAAAAACAGCGCGCGGGG |
| HTR+73 | Complement of +55 to +73 | CGCCTACGCCCTTCTCAGT |
a
Sequences corresponding to hTR are in uppercase; the T7 promoter (taatacgactcactataggg) is underlined; the first G of the triplet GGG is the first transcribed nucleotide (+1). Nucleotide mismatches in T7HTRmt+33 are denoted by lowercase italic letters.