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. 2021 Sep 7;22(5):1271. doi: 10.3892/etm.2021.10706

Figure 5.

Figure 5

TFEC activates AMPK/mTOR signaling in primary cardiomyocytes. (A) Western blotting showed that TFEC expression was significantly increased in primary cardiomyocytes transfected with Ad-TFEC compared with those transfected with Ad-NC. (B) Reverse transcription quantitative PCR showed that transfection with rAd-TFEC did not change the mRNA levels of AMPK, ACC and mTOR, compared with transfection with rAd-NC. (C) Western blotting showed that TFEC overexpression decreased the expression of p-AMPK and p-ACC but increased the level of p-mTOR. (D) Compound C significantly suppressed the activation of AMPK/ACC but increased the activation of mTOR, even in primary cardiomyocytes transfected with rAd-sh-TFEC. *P<0.05, **P<0.01 and ***P<0.001. AMPK, AMP-activated protein kinase; mTOR, mechanistic target of rapamycin; ACC, acetyl-CoA carboxylase; NC, negative control; p, phosphorylated; sh, short hairpin; TFEC, transcription factor EC.