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. 2021 Sep 13;22(5):781. doi: 10.3892/ol.2021.13042

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Copyright: © Du et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Ferroptosis levels in human RCC cells. (A) Representative western blots of GPX4 expression in human RCC cells, and quantification of the results. (B) Representative western blots of ACSL4 expression in normal renal proximal tubule cell line HK-2, and quantification of the results. Levels of (C) 5-HETE, (D) 12-HETE and (E) 15-HETE were detected in normal renal proximal tubule cell and RCC cells using commercial kits. *P<0.05 vs. control. GPX4, glutathione peroxidase 4; RCC, renal cell carcinoma; HETE, hydroxyeicosatetraenoic acid; 4; ACSL4, acyl-CoA synthetase long chain family member 4.