Figure 3.

Ferroptosis inhibits the proliferation of RCC cells by regulating ferroptosis. 786-O, A498 and Caki-1 and HK-2 cells were treated with 10 µM Lyc in the presence or absence of 1 µM ferrostatin-1, a ferroptosis inhibitor. (A-C) MDA levels in RCC cells. (D-F) ROS generation in RCC cells. (G-I) Representative western blots of GPX4 and ACSL4 expression in RCC cells, with quantification of the results. *P<0.05 vs. control; ^#P<0.05 vs. Lyc. RCC, renal cell carcinoma; MDA, malondialdehyde; ROS, reactive oxygen species; ACSL4, acyl-CoA synthetase long chain family member 4; GPX4, glutathione peroxidase 4; Lyc, lycorine.