Figure 3.

The impact of CC16 (club cell secretory protein) on airway hyperresponsiveness to methacholine challenge. (A) Wild-type (WT) and Cc16^−/− mice were infected with Mycoplasma pneumoniae (Mp) for 3 days, after which their airway resistance during methacholine challenge was determined on the Flexivent machine. n = 10 WT mice/group; n = 15 Cc16^−/− mice/group. (B) Recombinant CC16 (rCC16) given 2 hours before infection attenuated Mp-infected airway hyperresponsiveness in Cc16^−/− mice whether given via oropharyngeal (Oro) or i.v. routes; n = 9 Mp only; n = 6 Oro; n = 6 i.v. (C) The D67A mutation alters the activity of i.v. rCC16 to reduce airway hyperresponsiveness in Mp-infected CC16^−/− mice during Mp infection. n = 10–15 mice/group. (D) No differences in airway hyperresponsiveness were observed in Cc16^−/− vehicle control mice given either rCC16 or rCC16 D67A; n = 4 mice/group; **P < 0.01 at the respective dose indicated. Rrs = respiratory system resistance.