Table 2. H-bond array in the modeled 3D structures of Cin8 variants between the N-latch position and β10 of the NL and motor domain.
| Protein | Residue i | Atom | Residue J | Atom |
|---|---|---|---|---|
| wt Cin8 | F412 | O | F524 | N |
| F412 | O | S523 | Oγ | |
| F524 | N | S523 | Oγ | |
| G157 | O | G522 | N | |
| Cin8NLEg5 | F412 | O | K524 | N |
| G157 | O | N522 | Nδ | |
| G157 | O | N522 | N | |
| I414 | O | N522 | Nδ | |
| Cin8-G522N | F412 | O | F524 | N |
| G157 | O | N522 | N | |
| Cin8NLEg5-NG | F412 | O | K524 | N |
| G157 | O | G522 | N | |
| Cin8NLCut7 | G157 | O | N522 | N |
| Cin8NLCut7-NG | F412 | O | L524 | N |
H-bonds are based on geometric criteria. Here, we used stringent criteria: the distance between the donor and acceptor heavy atoms must be <0.36 nm, and the acceptor-donor hydrogen angle must be <30°.