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. Author manuscript; available in PMC: 2021 Sep 22.
Published in final edited form as: Cancer Res. 2020 Dec 22;81(4):885–897. doi: 10.1158/0008-5472.CAN-19-3219

Figure 2. TLR4 mediates LLC-induced p300 phosphorylation on Ser-12.

Figure 2.

(A) TLR4 is critical to p300 phosphorylation on Ser-12 and C/EBPβ acetylation on Lys-39 in LCM-treated myotubes. C2C12 myoblasts were transfected with siRNA specific for TLR4 or scrambled control siRNA (n = 3). Differentiated myotubes were treated with LCM for 2 h. Cell lysates were analyzed by Western blotting. (B) TLR4 is required for p300 phosphorylation on Ser-12 and C/EBPβ acetylation on Lys-39 in the muscle of LLC tumor-bearing mice. Wild type (WT) and TLR4−/− mice were implanted with LLC cells or injected with PBS as control (n = 6). In 21 days TA muscle were analyzed by Western blotting. * indicates a statistically significant difference (p < 0.05) determined by one-way ANOVA.