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. 2021 Sep 21;22:250. doi: 10.1186/s12931-021-01844-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Blocking increased Piezo1 expression inhibited RhoA/ROCK1 pathway activation in CS-treated HPMECs. A Western blotting showed Piezo1, ROCK1, RhoA, and GAPDH levels in ad-scramble + sham, ad-scramble + CS, ad-siRNA + CS, and ad-siRNA + sham cells. Quantitative analysis of Piezo1 B and ROCK1 E mRNA expression using qPCR in the above cells (n = 3). Quantitative analysis of Piezo1 C and ROCK1 F protein levels in the above cells (n = 3). D Active GTP-RhoA was assessed using a pull-down assay in the above cells (n = 3). G Quantitative analysis of ROCK1 mRNA expression using qPCR in the sham, ad-Yoda1, ad-Yoda1 + siRNA, and ad-siRNA cells (n = 3). H Quantitative analysis of ROCK1 mRNA expression using qPCR in sham, CS, ad-GSMTx4 + CS, and ad-GSMTx4 cells (n = 3). Data are shown as the mean ± SEM. *P < 0.05 versus the sham group and ^#P < 0.05 versus the scramble + CS or Yoda1 group. CS cyclic stretch, HPMEC human pulmonary microvascular endothelial cell, siRNA small interfering RNA, qPCR quantitative real-time PCR