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. 2021 Sep 21;22:250. doi: 10.1186/s12931-021-01844-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Inhibition of the RhoA/ROCK1 pathway did not affect the expression of Piezo1 in HPMECs. A Western blotting showed Piezo1, RhoA, and GAPDH levels in sham, CS, ad-fasudil + CS, and ad-fasudil cells. Quantitative analysis of RhoA B and Piezo1 C protein levels in the above cells (n = 3). D Western blotting showing ROCK1, Piezo1 and GAPDH level in sham, CS, ad-Y27632 + CS, and ad-Y27632 cells. Quantitative analysis of ROCK1 E and Piezo1 G protein levels in the above cells (n = 3). Quantitative analysis of ROCK1 F and Piezo1 H mRNA expression using qPCR in above cells (n = 3). Data are shown as the mean ± SEM. *P < 0.05 versus the sham group and ^#P < 0.05 versus the CS group. CS cyclic stretch, HPMEC human pulmonary microvascular endothelial cell, qPCR quantitative real-time PCR