Fig. 6.

Blocking increased Piezo1 expression attenuates VILI and improves survival in rats. A Western blotting and quantitative analysis of Piezo1 protein levels in the above groups of rats (n = 3). B Semi-quantitative analysis of lung tissues based on the lung injury score (n = 6). C Representative photomicrographs of lung tissues with H&E staining (original magnification × 40) in rats in the vehicle + sham, vehicle + VILI, GSMTx4 + VILI, and GSMTx4 + sham groups. D Lung edema determined based on the wet-to-dry lung weight ratio (n = 3), and E protein leakage from capillaries measured by Evans blue dye extravasation in rats (n = 6). F The levels of TNF-α, IL-1β, IL-6, and MCP-1 in BALF, as determined by ELISA (n = 6). G Survival rate of rats in the above groups (n = 10). Data are shown as the mean ± SEM. *P < 0.05 versus the vehicle + sham group and ^#P < 0.05 versus the vehicle + VILI group. VILI ventilator-induced lung injury, H&E hematoxylin and eosin, BALF bronchoalveolar lavage fluid, ELISA enzyme-linked immunosorbent assay