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. 2021 Sep 22;40:297. doi: 10.1186/s13046-021-02059-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PTK6 promotes CRC progression by activating JAK2/STAT3 signaling pathway. A The GSEA result indicates an enrichment of gene sets related to IL6 signaling pathway in PTK6 overexpression group. B Western blot assays indicate that IL6 can stimulate the activation of the PTK6-JAK2/STAT3 pathway in a dose-dependent manner in CRC cells. C The co-localization of JAK2 (green) and PTK6 (red) in CRC cells was assessed by IF staining. The scale bar represents 5 μm. D Co-IP results show an endogenous protein interaction between PTK6 and JAK2 in CRC cells. E Western blot assays show that the phosphorylation of PTK6 plays a vital role in the activation of the JAK2/STAT3 pathway. F The co-localization of p-STAT3 (green) and PTK6 (red) in vector, PTK6^WT, PTK6^KM and PTK6^YF overexpression CRC cells were assessed by IF staining. The scale bar represents 5 μm. G Western blot analyses show the expression of JAK2/STAT3 pathway members and stem cell markers in vector, PTK6^WT, PTK6^KM and PTK6^YF overexpression CRC cells with or without the treatment of RUXO. H Representative immunohistochemical (IHC) staining images of CRC tissues from the Nanfang cohort show the expression of PTK6, p-JAK2 and p-STAT3 in CRC and the adjacent normal tissues. Scale bars represent 50 μm. I Histograms show that the expression of PTK6 is correlated with that of p-JAK2 and p-STAT3 in CRC tissues