Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Haus6 | NCBI gene | Gene ID: 230376 | |
| Strain, strain background (M. musculus) | Nestin-Cre Haus6 cKO | This paper | See Materials and methods | |
| Strain, strain background (M. musculus) | Nestin-Cre Haus6 cKO Trp53 KO |
This paper | See Materials and methods | |
| Strain, strain background (M. musculus) |
Haus6 floxed Neo (Haus6fl-Neo) |
RIKEN http://www2.clst.riken.jp/arg/mutant%20mice%20list.html | CDB1218K | |
| Strain, strain background (M. musculus) | Haus6 floxed (Haus6fl) | RIKEN http://www2.clst.riken.jp/arg/mutant%20mice%20list.html | CDB1354K RRID:IMSR_RBRC09630 |
|
| Strain, strain background (M. musculus) | C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc | RIKEN (Kanki et al., 2006) |
RRID:IMSR_RBRC01834 | |
| Strain, strain background (M. musculus) | B6.Cg-Tg(Nes-cre)1Kln/J | Gift from Maria Pia Cosma (originally from Jackson Laboratories) | RRID:IMSR_JAX:003771 | |
| Strain, strain background (M. musculus) | Trp53-deficient mice (B6.129S2-Trp53tm1Tyj/J) | Jackson Laboratories | RRID:IMSR_JAX:002101 | |
| Antibody | Anti-α-tubulin (mouse monoclonal) | Sigma-Aldrich | #3873T | IF (1:500) |
| Antibody | Anti-acetylated α-tubulin (mouse monoclonal) | Sigma-Aldrich | #T6793 RRID:AB_477585 |
IF (1:500) |
| Antibody | Anti-βIII-tubulin (rabbit polyclonal) | Abcam | #ab18207 RRID:AB_444319 |
IF (1:1000) |
| Antibody | Anti-βIII-tubulin (mouse monoclonal) | BioLegend | #801201 RRID:AB_2313773 |
IF (1:1000) |
| Antibody | Anti-cleaved caspase-3 (rabbit monoclonal) | Novus Biologicals | #MAB835 RRID:AB_2243951 |
IF (1:500) |
| Antibody | Anti-BrdU (mouse monoclonal) | Abcam | #ab8955 RRID:AB_306886 |
IF (1:750) |
| Antibody | Anti-γ-tubulin (mouse monoclonal, clone TU-30) | ExBio | #ab27074 RRID:AB_2211240 |
IF (1:500) |
| Antibody | Anti-γ-tubulin (rabbit monoclonal) | Sigma-Aldrich | #T5192 RRID:AB_261690 |
IF (1:500) |
| Antibody | Anti-Ki67 (rabbit polyclonal) | Abcam | #ab15580 RRID:AB_443209 |
IF (1:750) |
| Antibody | Anti-nestin (mouse monoclonal) | Cell signaling | #4760 RRID:AB_2235913 |
IF (1:300) |
| Antibody | Anti-p53 (mouse monoclonal) | Cell signaling | #CST2524S RRID:AB_331743 |
IF (1:500) |
| Antibody | Anti-PAX6 (mouse monoclonal) | BioLegend | #901301 RRID:AB_2565003 |
IF (1:300) |
| Antibody | Anti-phosphorylated-Histone H3 (rabbit polyclonal) | Millipore | #06-570 RRID:AB_310177 |
IF (1:1000) |
| Antibody | Anti-TBR2 (rabbit polyclonal) | Abcam | #ab23345 RRID:AB_778267 |
IF (1:200) |
| Antibody | Anti-phospho-histone H2AX (Ser139) (mouse monoclonal, clone JBW301) |
Millipore | #05-636 RRID:AB_309864 |
IHC (1:500) |
| Antibody | Anti-p21 (rat monoclonal, HUGO291) |
Abcam | #ab107099 RRID:AB_10891759 |
IHC (1:500) |
| Antibody | Anti-centrin-Alexa 488 (rabbit polyclonal) | Homemade (Andrew Holland) (Phan et al., 2021) |
IF (1:500) | |
| Antibody | Anti-mouse IgG Alexa 488 (goat polyclonal) | Life Technologies | #A11029 RRID:AB_138404 |
IF (1:500) |
| Antibody | Anti-mouse IgG1 Alexa 488 (goat polyclonal) | Life Technologies | #A21121 RRID:AB_2535764 |
IF (1:500) |
| Antibody | Anti-mouse IgG1 Alexa 568 (goat polyclonal) | Life Technologies | #A21124 RRID:AB_2535766 |
IF (1:500) |
| Antibody | Anti-mouse IgG1 Alexa 633 (goat polyclonal) | Life Technologies | #A21052 RRID:AB_2535719 |
IF (1:500) |
| Antibody | Anti-mouse IgG2a Alexa 488 (goat polyclonal) | Life Technologies | #A21131 RRID:AB_2535771 |
IF (1:500) |
| Antibody | Anti-rabbit IgG Alexa 488 (goat polyclonal) | Life Technologies | #A11034 RRID:AB_2576217 |
IF (1:500) |
| Antibody | Anti-rabbit IgG Alexa 568 (goat polyclonal) | Life Technologies | #A11036 RRID:AB_10563566 |
IF (1:500) |
| Antibody | Anti-rabbit IgG Alexa 633 (goat polyclonal) | Life Technologies | #A21071 RRID:AB_141419 |
IF (1:500) |
| Antibody | Anti-mouse IgG HRP conjugated (goat polyclonal) | Dako-Agilent | #P0447 RRID:AB_2617137 |
IHC (1:500) |
| Antibody | Rabbit IgG polyclonal isotype control (rabbit polyclonal) |
Abcam | #ab27478 RRID:AB_2616600 |
IHC (1:500) |
| Antibody | Mouse IgG1 (NCG01) isotype control (mouse monoclonal) |
Abcam | #ab81032 RRID:AB_2750592 |
IHC (1:500) |
| Antibody | Mouse IgG2a isotype control (eBM2a) (mouse monoclonal) |
Invitrogen | #14-4724-82 RRID:AB_470114 |
IHC (1:500) |
| Sequence-based reagent | mAug6KO_FW | This paper | Genomic PCR primer Haus6 | 5′-CAACCCGAGCAACAGAAACC-3′ |
| Sequence-based reagent | mAug6KO_Rev | This paper | Genomic PCR primer Haus6 | 5′-CCTCCCACCAACTACAGACC-3′ |
| Sequence-based reagent | olMR1084 | This paper | Genomic PCR primer Cre | 5′-GCGGTCTGGCAGTAAAAACTATC-3′ |
| Sequence-based reagent | olMR1085 | This paper | Genomic PCR primer Cre | 5′-GTGAAACAGCATTGCTGTCACTT-3′ |
| Sequence-based reagent | olMR7338 | This paper | Genomic PCR primer control | 5′-CTAGGCCACAGAATTGAAAGATCT-3′ |
| Sequence-based reagent | olMR7339 | This paper | Genomic PCR primer control | 5′-GTAGGTGGAAATTCTAGCATCATCC-3′ |
| Software and algorithm | GraphPad Prism | GraphPad Software Inc | RRID:SCR_002798 | |
| Software and algorithm | QuPath | Queens University (Belfast,UK) | RRID:SCR_018257 | |
| Software and algorithm | FIJI (ImageJ) | NIH | RRID:SCR_002285 | |
| Other | Hematoxylin | Dako-Agilent | S202084 | |
| Other | 5-Bromo-2′-deoxyuridine (BrdU) | Sigma-Aldrich | B5002 | Injected peritoneally to pregnant females at a final concentration of 120 mg/kg of animal weight |
| Other | EnVision Flex Antibody Diluent | Dako-Agilent | K800621 | |
| Other | Envision Flex Wash buffer | Dako-Agilent | K800721 | |
| Other | 3-3′-diamino-benzidine | Dako-Agilent | K3468 | |
| Commercial assay or kit | Mouse on mouse (M.O.M) Immuno-detection Kit | Vector Laboratories | BMK-2202 RRID:AB_2336833 |