| Antibodies |
|
|
|
| APC anti-mouse CD45.1 |
BioLegend |
Cat# 110714 |
| Brilliant Violet 421 anti-mouse CD3e |
BioLegend |
Cat# 155617 |
| PerCP anti-mouse B220 |
BioLegend |
Cat# 103234 |
| Brilliant Violet 605 anti-mouse CD8a |
BioLegend |
Cat# 100744 |
| redFluor 710 anti-Human/Mouse CD44 |
Tonbo Biosciences |
Cat# 80–0441-U100 |
| FITC anti-mouse CD62L |
BioLegend |
Cat# 104406 |
| FITC anti-mouse CD122 (IL-2Rβ) |
BioLegend |
Cat# 123208 |
| PE anti-Mouse CD127 (IL-7Ra) |
Tonbo Biosciences |
Cat# 50–1271–U100 |
| PE/Cyanine7 anti-mouse/human KLRG1 |
BioLegend |
Cat# 138416 |
| Foxp3 / Transcription Factor Staining Buffer Kit |
Tonbo Biosciences |
Cat# TNB-0607-KIT |
| PE/Cyanine7 anti-mouse/human Eomes |
ThermoFisher Scientific |
Cat# 25–4875–82 |
| PE anti-mouse/human IRF4 |
ThermoFisher Scientific |
Cat# 12–9858–82 |
| Alexa Fluor 488 anti-mouse/human TCF1 |
Cell Signaling |
Cat# 6444S |
| APC anti-mouse IFN-γ |
BioLegend |
Cat# 505810 |
| PE anti-mouse IL-2 |
BioLegend |
Cat# 503808 |
| PE/Cyanine 7 anti-mouse TNF-α |
BioLegend |
Cat# 506324 |
| FITC anti-human/mouse Granzyme B |
BioLegend |
Cat# 372206 |
| MojoSort Mouse anti-APC Nanobeads |
BioLegend |
Cat# 480072 |
|
InVivomAb anti-mouse CD4 (GK1.5) |
BioXCell |
Cat# BE0003–1 |
| PE anti-mouse CD45.1 |
BioLegend |
Cat# 110708 |
| Alexa Fluor® 488 anti-mouse CD169 (Siglec-1) |
BioLegend |
Cat# 142419 |
| Alexa Fluor® 488 anti-mouse F4/80 |
BioLegend |
Cat# 123120 |
| Biotin anti-mouse CD3ε |
BioLegend |
Cat# 100304 |
| Cy5 AffiniPure Goat Anti-Mouse IgM, μ chain specific |
Jackson ImmunoResearch |
Cat# 115–175-075 |
| APC anti-mouse CD43 |
BioLegend |
Cat# 143208 |
| APC anti-mouse CD11c |
BioLegend |
Cat# 117310 |
| InVivoMAb anti-mouse CD40 (FGK4.5) |
BioXCell |
Cat# BE0016–2 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Streptavidin, Alexa Fluor 750 conjugate |
ThermoFisher Scientific |
Cat# S21384 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) |
ThermoFisher Scientific |
Cat# D1306 |
| ProLong Gold Antifade Mountant |
ThermoFisher Scientific |
Cat# P36934
|
| CellTrace Violet Cell Proliferation Kit |
ThermoFisher Scientific |
Cat# C34557
|
| Albumin from chicken egg white (ovalbumin) |
Millipore Sigma |
Cat# A5503 |
| Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126) |
BioLegend |
Cat# 927301 |
| Alu-Gel-S |
Serva |
Cat# 12261.01 |
| Ghost Dye Red 780 |
Tonbo |
Cat# 13–0865-T500 |
|
| Critical commercial assays |
|
|
|
| DyLight 650 Antibody Labeling Kit |
ThermoFisher Scientific |
Cat# 84535 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: C57BL/6J |
The Jackson Laboratory |
Cat# 000664 |
| Mouse: B6 CD45.1 |
The Jackson Laboratory |
Cat# 002014 |
| Mouse: OT-1 |
The Jackson Laboratory |
Cat# 003831 |
| Mouse: μMT−/−
|
The Jackson Laboratory |
Cat# 002288 |
| Mouse: MD4 |
The Jackson Laboratory |
Cat# 002595 |
| Mouse: sIgM− |
Boes et al., 1998
|
N/A |
| Mouse: AID−/−
|
Muramatsu et al., 2000
|
N/A |
| Mouse: H-2Kbm8
|
The Jackson Laboratory |
Cat# 023029 |
| Mouse: IL-27p28-GFP |
Kilgore et al., 2018
|
N/A |
| Mouse: IL-27p28−/− |
Janssen Research and Development |
N/A |
| Mouse: Ebi3−/− |
The Jackson Laboratory |
Cat# 008691 |
|
| Oligonucleotides |
|
|
|
| Poly(I:C) HMW (long synthetic analog of dsRNA) |
Invivogen |
Cat# tlrl-pic-5 |
|
| Recombinant DNA |
|
|
|
| SARS-CoV-2 spike vector |
Amanat et al., 2020
|
N/A |
|
| Software and algorithms |
|
|
|
| Prism version 9.1.2 |
GraphPad |
N/A |
| inForm version 2.5.1 |
Akoya Biosciences |
N/A |
| FlowJo version 10.7.1 |
BD Biosciences |
N/A |
