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. Author manuscript; available in PMC: 2021 Sep 22.
Published in final edited form as: Mol Cancer Res. 2020 Feb 6;18(5):774–786. doi: 10.1158/1541-7786.MCR-18-1370

Figure 1.

Figure 1.

EGFR activation promotes tyrosine-phosphorylation of CIC. A, HEK293 cells transfected with the indicated plasmids were lysed and immunoprecipitated (IP) with anti-HA antibody followed by immunoblotting with indicated antibodies. B, MEFs transfected with the indicated plasmids were lysed and immunoprecipitated with anti-HA antibody followed by immunoblotting with indicated antibodies. C, HEK293 cells endogenously tagged with HA-CIC, treated with or without EGF at indicated time points were lysed, and immunoprecipitated with anti-HA antibody followed by immunoblotting with indicated antibodies. D, NHA treated with or without EGF at indicated time points were lysed and immunoprecipitated with anti-CIC antibody followed by immunoblotting with indicated antibodies. WCE, whole-cell extract. Ctr, control.