Figure 3.

Four main differentiation trajectories were identified, and onset of lineage-affiliated transcriptional programs varied along “pseudotime” between tissues

(A–C) Force-directed graph (FDG) showing (A) 21 Louvain clusters, (B) superimposition of gene scores for 4 lineage gene sets, (C) pseudotime score from Monocle3 analysis. Dashed arrows represent the 4 main trajectories of differentiation from HSC/MPP toward lymphoid, erythroid, megakaryocytic, and myeloid progenitors.

(D, F, H, and J) Expression of canonical lineage-affiliated genes along pseudotime from HSC to erythroid, megakaryocytic, lymphoid, and myeloid trajectories for each tissue.

(E, G, I, and K) Expression of selected erythroid, megakaryocytic, lymphoid, and myeloid lineage-affiliated genes along pseudotime for each tissue.