TABLE 3.

Validation parameters for the analytes under investigation in hair

Compound	Mean regression curve and intercept	LOD (ng/mg)	LOQ (ng/mg)	Accuracy (% error)			Intra‐assay precision (% CV)			Inter‐assay precision (% CV)			Recovery (%) n = 15	Matrix effect (%) n = 15	Process efficiency (%) n = 15
				Low QC n = 15	Mid QC n = 15	High QC n = 15	Low QC n = 15	Mid QC n = 15	High QC n = 15	Low QC n = 15	Mid QC n = 15	High QC n = 15
N‐Deethyl‐dorzolamide	y = 0.0067x + 0.008	0.01	0.04	14.6	5.4	5.0	2.1	5.0	1.6	14.6	5.2	6.7	96.5	78.0	75.0
Dorzolamide	y = 0.0081x + 0.0511	0.01	0.02	9.5	4.6	1.6	0.8	4.5	0.9	12.4	4.7	2.1	97.0	92.7	90.1
Acetazolamide	y = 0.0075x + 0.0117	0.01	0.03	4.6	6.4	1.4	4.6	1.2	0.9	7.2	1.5	0.9	97.9	90.5	88.7
O‐Desmethyl‐brinzolamide	y = 0.0069x + 0.0433	0.05	0.15	4.4	9.1	1.7	5.1	6.1	0.8	6.2	5.6	1.5	97.4	91.2	89.0
Brinzolamide	y = 0.008x + 0.0322	0.02	0.06	2.1	6.5	3.5	1.6	3.0	1.7	1.9	5.0	4.2	99.0	96.6	95.6
N‐Acetyl‐dorzolamide	y = 0.0098x + 0.007	0.01	0.02	4.1	2.6	1.4	5.8	4.1	1.4	4.8	3.1	1.3	99.0	89.9	90.0

Note: Low, medium, and high quality control (QC) samples contained all standards at 0.5, 3.5, and 7.5 ng/mg, respectively. Analytical recovery, matrix effect, and process efficiency are displayed as mean value of low, medium, and high QC values.

Abbreviations: CV, coefficient of variation; LOD, limit of detection; LOQ, limit of quantification.