Figure 2. Triggered first Ca²⁺ sparks in pair.

A, probability of triggering Ca²⁺ spark with 2P photolysis of BIST‐2EGTA. Data are shown as means ± 95% confidence intervals; individual points represent average per animal. B–D, estimated parameters of first triggered Ca²⁺ sparks in WT and in mutant cells. R420Q control: N = 5, n = 17, first events = 710, first Ca²⁺ sparks = 511. WT control: N = 8, n = 26, first events = 1315, first Ca²⁺ sparks = 725; cAMP: N = 8, n = 24, first events = 815, first Ca²⁺ sparks = 637; caffeine: N = 3, n = 12, first events = 666, first Ca²⁺ sparks = 458; CPA: N = 8, n = 25, first events = 1452, first Ca²⁺ sparks = 867. Horizontal black lines represent significant difference, P < 0.05.