Figure 6.

Pharmacologic inhibition of AXL by R428 treatment impairs lung fibroblast cell viability. (A) Cells were incubated with various concentrations of R428 (bemcentinib) for 72 hours and then subjected to MTT assays (n = 4). Data are expressed as means ± SD; *P < 0.05 versus PBS-2. (B and C) Lung fibroblasts were exposed to various concentrations of R428 as indicated above. After 72 hours of treatment, cell viability was determined by MTT assays. The IC50 value of R428 for all tested lung fibroblast cells was shown (B and C). (D) Smoke-exposed cells (CSE-2) were treated with 2.5 μM R428 for 24 hours and then subjected to Western blot analysis. (E) R428 treatment in cells with exposure to short-term and long-term CSE. Cells were exposed to PBS (control) or 10% CSE (smoke) for 3 days (left) or 21 days (right). These cells were subjected to the indicated doses of R428. After 72 hours of treatment, cell viability was determined by MTT assays. Data are expressed as means ± SD; *P < 0.05 versus control (n = 3). IC50 = half maximal inhibitory concentration.