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. Author manuscript; available in PMC: 2021 Sep 22.
Published in final edited form as: Pharm Res. 2019 May 15;36(7):101. doi: 10.1007/s11095-019-2634-3

Fig. IV. Comparison of transport function between V174A- and WT-OATP1B1 in transiently transfected HeLa cells.

Fig. IV.

A. FLAG immunoblot in FLAG-WT- and FLAG-V174A-OATP1B1-expressing HeLa cells. HeLa cells were transfected with an expression vector encoding FLAG-WT-, FLAG-V174A-OATP1B1, or Fugene HD reagent alone. Forty-eight hours post-transfection, FLAG immunoblot was conducted with β-actin as the loading control. The mixed-effect model estimated fold change and associated SE in FLAG-OATP1B1 expression of V174A-OATP1B1 vs. WT-OATP1B1 control was expressed as mean ± SE (n=3 in duplicate). B. Mixed-effect model-estimated fold change and associated SE of [3H]E217βG (1 μM, 2 min) of V174A-OATP1B1 vs. WT-OATP1B1 (*, p<0.05, n=3, in triplicate). In each individual experiment, [3H]E217βG accumulation mediated by WT- or V174A-OATP1B1 was normalized by relative OATP1B1 protein levels in whole cell lysates.