FIGURE 2.

Multiple snRNA gene variants are transcribed in human cells. (A) Expression of snRNA variants in pooled adult and fetal tissues relative to the respective canonical gene (2^−ΔCt) using RT-qPCR. U6 snRNA was normalized to canonical U4 snRNA. Error bars represent the standard error of the mean (SEM) of three technical replicates. The x-axis is log₁₀ scaled. (B) Fold differential expression of snRNA variants in pooled adult versus fetal tissues. Normalized ratios of expression between adult and fetal tissues are displayed (2^−ΔΔCt). Ratios >1 indicate greater relative expression of the snRNA in adult tissues. Ratios <1 indicate greater relative expression in fetal tissues. (C) Expression of snRNA variants in 293T cells relative to the respective canonical gene (2^−ΔCt) using RT-qPCR. U6 snRNA was normalized to canonical U4 snRNA. (See Supplemental Fig. 4A for expression of a subset of snRNAs relative to U1-1.) Error bars represent the standard error of the mean (SEM) of three technical replicates from human 293T cell RNA. The x-axis is log₁₀ scaled. (D) Heat map of the fold differential expression of the snRNA genes listed at left across the human cell lines listed at top, as determined by RT-qPCR and indicated by the color scale below. Cell lines were hierarchically clustered based on their snRNA expression profiles (bottom). Three technical replicates were run for each sample.