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. 2021 Oct;27(10):1186–1203. doi: 10.1261/rna.078768.121

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© 2021 Mabin et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Expressed snRNA variant genes exhibit sequence variation in RNA:RNA and RNA:protein interacting domains. Arrows show the positions of selected nucleotide variants relative to the canonical snRNA. Bold letters show the substituted base, followed by the variant snRNA(s) in which the substitution occurs. Colored regions within each structure are known functional sequences further described in Supplemental Figure 5. snRNA genes: (A) U1, (B) U2, (C) U4, (D) U5. (E) Predicted 3′ stem–loop structure of U5 snRNA variants with conserved or divergent base changes. Bold letters show the substituted base as compared to U5A. The free energy of formation (ΔG) and snRNA half-lives (t_1/2) are shown below each snRNA variant structure. For reference, the free energy of formation for the 3′ stem of U5A-1 is −13.53 kcal/mol. Structures and free energy of folding were calculated using the Vienna RNAfold webserver (rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi).