Fig. 5.

Single-cell nucleic acid analysis on valve-based platform: A-1 schematic of membrane-based valve, A-2 schematic of single-cell nucleic acid analysis with valve-based microfluidic chip, B integrated chip for high throughput analysis with intensively integrated valves, reactors, and channels, C fluidic control on valve-based platform (Thorsen et al. 2002), D different working mechanisms of membrane-based valve (Li et al. 2019b; Sun et al. 2020), E integrated Fluidigm C1 system based on the concept of valve control (Pollen et al. 2014)